Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

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Genome-wide identification of transcription factors and transcription-factor binding sites in oleaginous microalgae Nannochloropsis

Nannochloropsis spp. are a group of oleaginous microalgae that harbor an expanded array of lipid-synthesis related genes, yet how they are transcriptionally regulated remains unknown. Here a phylogenomic approach was employed to identify and functionally annotate the transcriptional factors (TFs) and TF binding-sites (TFBSs) in N. oceanica IMET1. Among 36 microalgae and higher plants genomes, a...

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Safety assessment of the microalgae Nannochloropsis oculata

Nannochloropsis oculata is a marine-water microalgae that is considered to be a good source of omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), utilized in the production of an omega-3 oil for use as a dietary supplement. This study investigates the safety of N. oculata in male and female Sprague-Dawley rats administered a 0 or 10 mL/kg bw/rat N. oculata (10E8 viable cells/mL) sus...

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BACKGROUND The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO2 for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor. RESULTS We systematically characterized endogenous genetic elements of Synechocystis 6803, includin...

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Lipid Profile Remodeling in Response to Nitrogen Deprivation in the Microalgae Chlorella sp. (Trebouxiophyceae) and Nannochloropsis sp. (Eustigmatophyceae)

Many species of microalgae produce greatly enhanced amounts of triacylglycerides (TAGs), the key product for biodiesel production, in response to specific environmental stresses. Improvement of TAG production by microalgae through optimization of growth regimes is of great interest. This relies on understanding microalgal lipid metabolism in relation to stress response in particular the depriva...

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ژورنال

عنوان ژورنال: Plant Cell Reports

سال: 2018

ISSN: 0721-7714,1432-203X

DOI: 10.1007/s00299-018-2270-0